FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004683890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927931
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTCGAC34770.3747045847158894No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29330.31607953608619604No Hit
GGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGC28690.3091824715415263No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAAT26660.2873058449389017No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25910.2792233474256168No Hit
GGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC25120.27070978337829No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25060.2700631835772272No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA23500.2532515887495945No Hit
GTGTATTACTGTGCGAGAGGTCGTCCTTTCACATTTTTCCGTGAGACAAG21970.23676329382249328No Hit
GTAATACACAGCCGTGTCTTCGGCCTTCAGACTGTTCATTTGTAGATACACTGAACTCTTGGCGTCGTCTCTGGA21570.23245262848207462No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC20400.21984393236135014No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACT19260.20755853614115705No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC18770.20227797109914422No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC18650.20098477149701863No Hit
GGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAT18190.1960275063555372No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT17760.19139354111458717No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC17450.18805277547576274No Hit
CCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGA17440.18794500884225226No Hit
CCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAC17290.1863285093395953No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCAC17130.18460424320342783No Hit
CCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCCTCAGGGATCCCT16880.1819100773656662No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16800.18104794429758247No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16780.18083241103056152No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTG16780.18083241103056152No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16620.17910814489439408No Hit
GAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTG16480.17759941202524757No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16110.17361204658536034No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC15910.17145671391515102No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15170.16348198303537656No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15100.1627276166008033No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG15000.16164995026569864No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14930.16089558383112537No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT14530.15658491849070674No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGC14320.15432181918698695No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCACCATCATCAT14210.15313638621837183No Hit
GCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA14080.1517354199827358No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13970.15054998701412067No Hit
GACCATCAACAGGGTCGAAGCCGGTGATGAGGCCGACTATTATTGTCAGGTGTGGGATAGTAGTGGTGACCAT13900.1497956205795474No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTA13790.14861018761093228No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13630.14688592147476484No Hit
CTTTTAGTTCCGATGTTGTTCCCCGCACAGATAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG13610.1466703882077439No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAACAGGGTCGA13610.1466703882077439No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13560.14613155504019157No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACA13430.14473058880455553No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13410.14451505553753458No Hit
GTTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCACC13340.14376068910296133No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAGTT13020.14031215683062642No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCC12930.13934225712903223No Hit
CCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAATAATAGTCGGCCTCAT12680.13664809129127056No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAATA12660.13643255802424964No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTCGACCCTGTTGATGGTCAGGGTGGCCGTGTTCCC12650.13632479139073916No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG12620.1360014914902078No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12570.13546265832265544No Hit
CGGCCACCCTGACCATCAACAGGGTCGAAGCCGGTGATGAGGCCGACTAT12470.1343849919875508No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC12380.1334150922859566No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGATCCACGTCAGCCAAAAAATTTCAAAGGGGAATCCAGAGGCTGCAC12330.13287625911840428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12150.1309364597152159No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12120.13061315981468452No Hit
CTCCTGTGCAGCCTCTGGATTCCCCTTTGAAATTTTTTGGCTGACGTGGATCCGCCAGGCTCCAGGGAAGGGG11950.12878112704500658No Hit
GGCCAGGCCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCC11790.1270568609088391No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11710.1261947278407554No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG11520.12414716180405656No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTC11470.12360832863650421No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT11400.12285396220193096No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAA11210.1208063961652321No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA11180.1204830962647007No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACC11090.11951319656310652No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA11070.1192976632960856No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11050.11908213002906466No Hit
TCTATGATGATGGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC10910.11757339715991813No Hit
GTGTGGGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAA10840.11681903072534487No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10800.11638796419130301No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10790.11628019755779255No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC10700.11531029785619837No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10570.1139093316205623No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC10460.11272389865194718No Hit
GTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT10460.11272389865194718No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGT10400.1120772988508844No Hit
GTATTACTGTGCGAGAGGTCGTCCTTTCACATTTTTCCGTGAGACAAGTGGTTATGCCAGTTTTGACTACTGGGG10300.11099963251577973No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10220.110137499447696No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10190.1098141995471646No Hit
CAGTAATACACAGCCGTGTCTTCGGCCTTCAGACTGTTCATTTGTAGATA10150.10938313301312275No Hit
GAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCC10080.10862876657854949No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCC9990.10765886687695528No Hit
CTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG9930.1070122670758925No Hit
GTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGG9920.10690450044238203No Hit
CTATTATTGTCAGGTGTGGGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCT9880.10647343390834017No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9870.1063656672748297No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT9850.10615013400780877No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9790.10550353420674598No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAA9710.10464140113866224No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCCTCAGGGATCCCTG9640.10388703470408898No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9330.10054626906526455No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9290.10011520253122269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)