FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108169
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGCTCCAACATCGGGACAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC49210.4440658419428805No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGGCTGCTGTCATAGGACTGGCAGTAAT48710.43955389475792955No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGGCTGCTGTCATAGG47120.42520590270978526No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44690.4032778393909232No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAGCAATCGG40070.3615874474019757No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39160.35337570352536485No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35140.3170996481583585No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA34970.31556558611547514No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34940.3152948692843781No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC34430.310692683155728No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAGCAATCGGCCCTC31120.2808235927913522No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30780.27775546870558554No Hit
ATACCACACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC29730.26828037961718837No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29280.26421962715073244No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28210.2545640601749372No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC27270.24608159946722927No Hit
GTCCTATGACAGCAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG25590.2309214569257938No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT25550.23056050115099772No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC25220.22758261600893007No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24780.22361210248617314No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC24380.22000254473821232No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATA24250.21882943847012504No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23330.21052745564981512No Hit
ATGCTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG23230.20962506621282495No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22800.20574479163376708No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG22500.20303762332279643No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC22490.2029473843790974No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT22480.2028571454353984No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC22160.19996949923702972No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTG21960.1981647203630493No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG21950.1980744814193503No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT20850.18814819761245802No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGACAGGTTATGATGTACACTGGTACC20570.18562150718888545No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC20280.18300457782161383No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20240.18264362204681778No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA19720.1779511969744687No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCT19660.17740976331227457No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19600.17686832965008045No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA18910.17064184253484804No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18520.16712252373058623No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG18470.1666713290120911No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG18370.16576893957510092No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17980.1622496207708391No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG17770.16035460295315968No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC17570.15854982407917925No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17440.157376717811092No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT17360.15665480626149983No Hit
CTCCTCATCTATGCTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTC17080.15412811583792724No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT17020.15358668217573312No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC16850.1520526201328498No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC16360.14763091189159777No Hit
ACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGG16280.1469090003420056No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC16000.144382309918433No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC15710.14176538055116142No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15690.1415849026637634No Hit
GTGTACATCATAACCTGTCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCC15470.13959964590238494No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15460.1395094069586859No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT15310.1381558228032006No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT15290.13797534491580254No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15060.13589984921072507No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCC14910.13454626505523978No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAG14810.13364387561824956No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC14680.1324707693501623No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGACAGGTTATGATGT14640.13210981357536622No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14550.13129766308207502No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA14210.12822953899630832No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG14040.12669547695342498No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14040.12669547695342498No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13710.12371759181135729No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCA13690.12353711392395926No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC13670.1233566360365612No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13530.12209329082477492No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG13470.1215518571625808No Hit
GCCCCCAAACTCCTCATCTATGCTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT13420.1211006624440857No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13340.12037875089449353No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC13320.1201982730070955No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG13320.1201982730070955No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13180.11893492779530919No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT13100.11821301624571703No Hit
CCCCTGAGGGCCGATTGCTGTTAGCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGT13030.1175813436398239No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13020.11749110469612488No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTC12990.11722038786502781No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT12940.1167691931465327No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTGTGGTATTCGGCGG12650.1141522637792611No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC12550.1132498743422709No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12440.11225724596158168No Hit
GTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACCTGTCCCGATGTT12420.11207676807418364No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12400.11189629018678558No Hit
GCCGAATACCACACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCC12280.11081342286239734No Hit
GATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACCTGTCCCGATGTTGG12240.11045246708760127No Hit
CATCATAACCTGTCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGAC12230.11036222814390223No Hit
CTGTTAGCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACCTGTCCCG12190.11000127236910616No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCC12130.10945983870691203No Hit
CCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGACAGGTTATGATGTACACTGGT12070.10891840504471792No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11900.10738434300183455No Hit
GCTTGGTCCCTCCGCCGAATACCACACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCT11830.1067526703959414No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT11750.10603075884634924No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG11740.10594051990265023No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT11720.10576004201525219No Hit
GTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACCT11630.10494789152196099No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC11610.10476741363456296No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11600.10467717469086395No Hit
GCTCCAACATCGGGACAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCT11220.10124809483030114No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC11170.10079690011180606No Hit
GTCAGGGACCCCTGAGGGCCGATTGCTGTTAGCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGT11130.10043594433700997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2900.075.7782970
CTAATAG200.007041977352.3293956
TTTAAGT4000.051.70753570
GGTATCA9200.050.4964451
ACTGGTG32950.037.27022670
TATACAC1950.036.5135463
TCTCGTA1600.036.2947838
ATTCCGG2854.5307388E-836.28598870
CGTATGC1800.036.195841
GTATCAA23900.035.0450861
ACGAGAC1950.034.77166722
CTTATAC2050.034.73711
TCGTATG1700.034.25011440
ATAGCGG400.002735292534.1349637
TCTCGCG6450.034.07085870
TATCAAC25600.032.448562
TGACAGT28700.032.42981370
ATGTGAT4007.6215656E-1032.3172170
GCGCGTG7250.032.09433470
CACGAGA2150.031.5356721
Produced by FastQC (version 0.11.5)