FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32951572
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA484850.14714017285730707TruSeq Adapter, Index 16 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN371280.11267444236044337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG105650.056.02814545
TATGCCG108500.054.75181648
CGTATGC109950.054.09093546
CTCGTAT108550.051.96902544
GATCGGA118100.051.199991
ACACGTC126100.048.33717713
CGTCCCG124600.047.36091635
ACCCGTC125500.047.1303232
ATCGGAA132450.046.326252
ATGCCGT130000.045.8581249
CCGATCT114700.045.45231239
TCCCGAT121700.045.03533637
GTCCCGA129900.043.7970236
TCTCGTA125500.043.45380443
CCCGATC121000.043.34390638
ACGTCTG140000.043.21516815
CACCCGT141000.041.99798631
ATCTCGT127950.041.3175842
GCCGTCT145900.040.7434151
CACGTCT150800.040.28167714
Produced by FastQC (version 0.11.5)