FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34206056
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2271110.6639496818925865TruSeq Adapter, Index 13 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1269910.37125297345008146No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT555580.16242153143876042TruSeq Adapter, Index 13 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG346240.10122184212058823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA366800.063.190141
ATCGGAA393000.061.4513362
TCGGAAG411900.058.502323
TCGTATG421200.056.5792245
TATGCCG423050.056.22033348
CGTATGC424600.056.10275746
CTCGTAT419250.055.38266444
ACACGTC443800.054.88141313
ATGCCGT443100.053.6064349
ACGTCTG454250.053.44309615
CGGAAGA452100.053.393194
TCTCGTA435550.053.2051243
AATCTCG437550.052.53170841
ATCTCGT441900.052.2825242
CACGTCT467250.052.09762614
GCCGTCT457800.051.46237651
TGCCGTC466850.050.8060650
CACACGT480200.050.79380812
CCGTCTT469000.050.18984252
CGTCTGA489700.049.60968816
Produced by FastQC (version 0.11.5)