FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27193735
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG42150.039.91905643
CGTATGC44850.038.756644
TATGCCG45350.037.9480246
GATCGGA52050.037.084181
CTCGTAT38600.031.79115342
ATCGGAA63200.031.2403412
ACACGTC65350.030.2560413
GTCGTAT19550.026.49466342
ACGTCTG79950.024.77423115
ATGCCGT67850.024.64702447
TCTCGTA51350.023.9643141
TCGGAAG85050.023.7851123
CACGTCT85400.023.19316514
CGTCTGA88550.022.64280716
ATCTCGT60000.020.50873640
CACACGT99200.020.00183512
GCCGTCT82250.019.6990349
GTATGCC92850.019.58239645
TGCCGTC89600.019.13040548
CCGTCTT87500.018.91476450
Produced by FastQC (version 0.11.5)