FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25646379
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA295159511.508817677536467TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT7011022.73372704973283TruSeq Adapter, Index 19 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN817220.31864927208632454No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400850.15629886776608895No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA300890.1173226052691493TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA285340.11125937115723042TruSeq Adapter, Index 19 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA279430.10895495227610882TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAA258070.10062629114230902TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG635450.071.6484770
GATCGGA4254900.068.942211
ATCGGAA4375750.068.7745742
TCGGAAG4387450.068.525943
ACACGTC4385900.068.4844713
ACGTCTG4383000.068.3279515
CACGTCT4391200.068.2441514
CACACGT4408300.068.1476112
GCACACG4407600.068.1411411
CGGAAGA4419500.068.118524
CGTCTGA4396850.068.1166516
TCGTATG4341100.068.0382845
TATGCCG4339350.068.0021948
CTCGTAT4198750.067.9878544
CGTATGC4348450.067.9854746
AGTCACG4380000.067.9104228
GTCACGT4375600.067.85995529
TGAAACG4364350.067.8520935
ATGCCGT4351050.067.7834649
GAAACGA4308400.067.7754436
Produced by FastQC (version 0.11.5)