FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20965847
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1398690.6671278293693549TruSeq Adapter, Index 3 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN437600.20872040132697717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG188150.061.6650243
TATGCCG190750.061.45613546
CGTATGC191350.061.30959344
GATCGGA200900.061.2799451
CTCGTAT181500.061.16280442
ATCGGAA216650.059.539842
ACACGTC218600.058.63684513
TCTCGTA192300.056.98254841
ATGCCGT206500.056.14906747
ACGTCTG231450.055.3660815
CACGTCT233500.054.89526714
TCGGAAG235550.054.8930443
ATCTCGT201900.054.4420640
CGTCTGA236450.054.12158216
CACACGT239700.053.649412
GCCGTCT214300.052.48907549
CCGTCTT215700.052.4737850
ACTTAGG246050.051.9070632
CTTAGGC244250.051.89415433
CATCTCG212700.051.8215939
Produced by FastQC (version 0.11.5)