FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23261498
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN577940.2484534744924854No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC240510.10339402905178334TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG42500.044.24926843
TATGCCG45850.042.3832846
CGTATGC45550.042.27927844
GATCGGA52100.041.9231341
CTCGTAT47200.040.43200742
TATCTCG54000.036.6909739
ATCGGAA60500.036.379952
ACCGATG62650.035.06154632
CGATGTA62750.035.00687834
ACACGTC63650.035.0010913
CACCGAT63650.034.67406531
TCTCGTA58550.033.1277541
CCGATGT69400.031.9022733
ATCTCGT62650.031.56968340
ACGTCTG71400.031.2506315
ATGCCGT66150.028.90411247
TCACCGA77400.028.82853530
CACGTCT78900.028.45629914
GTCACCG78350.028.16816329
TCGGAAG82900.026.9675373
Produced by FastQC (version 0.11.5)