FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28951469
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC19140866.611360549614943TruSeq Adapter, Index 9 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8743903.0201921705596355No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC769440.26576889759894395TruSeq Adapter, Index 9 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG311570.10761802794877179No Hit
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT299490.10344552810083663TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2348100.085.63914570
GATCGGA2195300.068.486451
ATCGGAA2342650.068.1303562
TCGGAAG2349750.067.716693
CGGAAGA2379600.066.9685754
ACACGTC2460200.065.18455513
TCGTATG2447950.064.71223443
ACGTCTG2468000.064.7120715
CACGTCT2475000.064.6458714
CTCGTAT2386300.064.58542
GTCACGA2462850.064.5793929
CACACGT2485600.064.5774212
GCACACG2492250.064.4585811
AGTCACG2470400.064.44728
TCACGAT2475300.064.2594530
CACGATC2473500.064.2442231
ACGATCA2479200.064.2056432
CGATCAG2480550.064.13270633
TCTCGTA2380000.064.1158141
ATCTCGT2359200.063.9832740
Produced by FastQC (version 0.11.5)