FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23606789
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA415330.17593667652131764TruSeq Adapter, Index 13 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN365810.1549596601215015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG81950.050.98343745
GATCGGA82500.050.0104941
CGTATGC83650.049.9485846
TATGCCG84750.049.59149648
CTCGTAT84700.047.5621744
ATCGGAA92550.046.6059462
ACACGTC97050.044.7521513
TCTCGTA94400.042.8215143
AATCTCG94000.042.6689541
ATCTCGT97850.041.3460942
ATGCCGT104300.040.46449
ACGTCTG109000.039.7503815
CACGTCT112150.038.5719314
TCGGAAG113400.038.3433653
CGTCTGA115400.037.51550316
GCCGTCT116100.036.38387351
CCGTCTT119600.035.11705852
CACACGT124650.034.95470412
TGCCGTC125700.033.4933850
GCACACG132200.033.1950411
Produced by FastQC (version 0.11.5)