FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31984888
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2259570.7064492456562612TruSeq Adapter, Index 10 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1886160.5897034874719587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)