FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25514340
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN259760.1018094138433524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44900.044.36364745
TATGCCG48200.041.4033848
CGTATGC50100.040.0380846
ACGTCCG51900.039.2368532
CTCGTAT51000.038.5109744
GATCGGA52550.038.247631
ACACGTC65150.031.67928713
AGTCACG64950.031.67164628
ATCGGAA64600.031.5281722
TCTCGTA63700.031.32348643
CGCACAT66150.030.68229137
CGTCCGC68100.030.0565933
ATGCCGT67500.029.87576949
GTCCGCA69150.029.24864634
GTCACGT71500.029.01302129
ATCTCGT70800.028.27943442
ACGTCTG75300.027.50133515
TCACGTC76800.026.55894930
CGTCTGA81350.025.3704916
GCCGTCT79400.025.00577451
Produced by FastQC (version 0.11.5)