FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25729617
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG40700.035.7874843
CGTATGC44350.033.3918944
TATGCCG45900.031.8886946
CTCGTAT42350.031.35459142
GATCGGA53000.028.7364541
ATCGGAA57250.027.3773172
ACACGTC60800.026.45642513
ATGCCGT60900.024.14954647
TCTCGTA58700.022.97543341
AATCTCG59550.022.9374239
ACGTCTG71150.022.46143715
ATCTCGT64750.021.04264540
CGTCTGA79200.020.39771516
GCCGTCT73250.019.93749849
CACGTCT80700.019.84633414
TCGGAAG81150.019.8272653
CACACGT87050.018.67787412
CCGTCTT78550.018.54892750
CGCGCTA18350.017.62033546
TGCCGTC83350.017.35404448
Produced by FastQC (version 0.11.5)