FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22537709
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC928000.4117543624332003TruSeq Adapter, Index 5 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN897860.398381219670553No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC320820.14234809758170186TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG152550.062.18997243
TATGCCG158500.061.46227346
CGTATGC158800.061.2108444
GATCGGA180000.060.3738481
ATCGGAA186500.059.033732
CTCGTAT122450.057.8575442
ACACGTC189300.057.6214313
ACGTCTG199000.054.56864515
ATGCCGT176350.054.35660647
TCTCGTA130500.053.96708741
TCGGAAG205250.053.6207163
GTCGTAT47600.053.3126742
CACGTCT204300.053.2719614
ATCTCGT135600.052.34576840
CGTCTGA208800.051.99106216
GCCGTCT181950.050.7183449
CACACGT216050.050.56762712
CCGTCTT190300.049.2628550
TGCCGTC194750.048.8114548
GTATGCC202250.048.6292645
Produced by FastQC (version 0.11.5)