FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23910651
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC430420.18001182820158262TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG62100.053.1741143
TATGCCG65350.050.7434646
CTCGTAT65850.049.93367842
CGTATGC66950.049.53004544
GATCGGA68950.048.9257551
TATCTCG72850.045.4678439
ATCGGAA76000.044.788212
GTCACGC76300.044.18126329
CGCCAAT78250.043.4370233
ACACGTC79100.043.0565913
AGTCACG79200.043.0023228
TCTCGTA81600.040.7211941
ATGCCGT83650.040.0172947
ACGTCTG87300.039.01265715
ACGCCAA88100.038.6981732
ATCTCGT87750.037.74800540
GCCGTCT89950.036.8678849
TCGGAAG94200.036.648613
CGTCTGA95050.035.9049216
CACGTCT96950.035.23681614
Produced by FastQC (version 0.11.5)