FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9332877
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2139232.2921442123366673TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG529730.567595608513859No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN511150.5476874922920338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG283000.079.4306870
GATCGGA229700.066.9959261
ATCGGAA233350.066.111472
TCGTATG232300.065.9442443
CGTATGC234100.065.4414244
TATGCCG234350.065.4228346
CTCGTAT232250.065.40066542
ACACGTC238400.064.60580413
TCTCGTA236100.064.2304441
ACGTCTG240200.064.0207415
TCGGAAG244050.063.454723
ATCTCGT238550.063.45143540
ATGCCGT242250.063.2770247
CACGTCT246500.062.412714
CGTCTGA248000.062.00684416
GCCGTCT249350.061.52440349
CCGTCTT250100.061.35877250
CACACGT251750.061.2212512
GTATGCC251300.061.03481745
TGCCGTC252250.060.77113348
Produced by FastQC (version 0.11.5)