FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9742110
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG742530.7621860151445632No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN408430.41924182748911687No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC185380.19028731968741885TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26650.049.0548143
CTCGTAT27350.047.9246942
CGTATGC27950.047.14832344
TATGCCG29100.045.8853446
GATCGGA29550.044.827321
TCTCGTA31700.041.5678441
ATCGGAA32100.041.3660472
AATCTCG32200.040.7030739
ACACGTC33950.039.511513
ATCTCGT34350.037.85355840
ACGTCTG38000.035.02641315
ATGCCGT39150.034.19710547
CACGTCT40150.033.41026714
GCCGTCT42150.031.43524749
TCGGAAG45150.029.9485193
CCGTCTT44300.029.83218650
CACACGT45250.029.56804712
CGTCTGA45600.029.26458516
TGCCGTC48450.028.06403448
TTGAATC48150.027.8659936
Produced by FastQC (version 0.11.5)