FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7219865
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN819651.1352705348368701No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC676520.9370258308153961TruSeq Adapter, Index 10 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG482780.6686828631837299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA80750.062.1649131
AAAAGGG119800.061.9401770
ATCGGAA83300.060.625182
TCGTATG82500.060.3465443
CTCGTAT83250.059.67591542
TATGCCG84500.058.89069746
CGTATGC85050.058.50248344
TATCTCG84650.058.47377439
ACACGTC86550.058.0500813
TCTCGTA86850.057.07901841
TAGCTTA88800.056.1981734
ATCTCGT89700.055.3794140
ACGTCTG90650.055.3095515
TCGGAAG91650.055.215543
ATGCCGT91050.054.81118447
CACGTCT92300.054.3584514
ACTAGCT92400.054.041732
GTCACTA93000.053.8357129
CACTAGC94050.053.1283131
TCACTAG94550.052.99250430
Produced by FastQC (version 0.11.5)