FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13322181
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG956900.7182757838224837No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN425010.3190243399335289No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA379380.28477319141663066TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA58850.053.174631
ATCGGAA61600.050.6199842
TCGTATG71450.044.86584545
CTCGTAT73350.043.2277144
CGTATGC74550.043.0941246
TATGCCG75450.042.4904748
TCGGAAG75300.041.7792473
TCTCGTA80000.039.6769743
ACACGTC83350.039.31583813
AATCTCG82400.038.60426341
ATCTCGT82800.038.5023242
ACGTCTG86700.037.47620415
ATGCCGT88950.036.237849
CACGTCT90600.036.169714
CGTCTGA94000.034.7506616
CACACGT95050.034.4397712
CCGTCTT95000.034.1170252
CGGAAGA92750.034.031464
GCCGTCT96950.033.42942451
GTATGCC99900.032.33394247
Produced by FastQC (version 0.11.5)