FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12362518
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN981680.7940777113529784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG902260.7298351355282152No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA535890.4334796519608708TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC67050.057.50981546
TCGTATG67850.056.88089445
CTCGTAT67800.056.66550444
TATGCCG68400.056.4288148
GATCGGA68700.056.3820951
ATCGGAA71600.054.3780752
ACGTCCG72050.053.5428632
TCTCGTA73850.052.16369243
CGCACAT75450.050.72583437
ACACGTC77600.049.93452513
AGTCACG79400.048.84587528
ATCTCGT78850.048.81002842
GTCCGCA80200.048.2790634
ATGCCGT82300.047.11128649
TCGGAAG83200.047.04763
ACGTCTG82850.046.89594315
GTCACGT83550.046.54406429
CGTCCGC83250.046.4666333
TCACGTC83900.046.1853530
CACGTCT84500.045.81590314
Produced by FastQC (version 0.11.5)