FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25940383
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2086770.8044484154301037TruSeq Adapter, Index 13 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN963970.37160977923880306No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT476960.1838677555377652TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA331200.063.2433661
ATCGGAA345050.061.8506132
TCGTATG350300.058.92499545
TATGCCG350850.058.75414348
CGTATGC354050.058.3533646
CTCGTAT348750.058.32730544
TCGGAAG368900.057.782693
ACACGTC377700.056.67951213
TCTCGTA359850.056.54449543
ATGCCGT372450.055.3033849
ACGTCTG387900.055.00115215
AATCTCG369550.054.89488641
ATCTCGT371800.054.602542
CGGAAGA393350.054.3235054
CACGTCT396350.053.94196714
GCCGTCT381400.053.57384551
CGTCTGA400800.053.32623716
CACACGT406050.052.70438412
CCGTCTT390400.052.40327552
GTATGCC397750.051.97072247
Produced by FastQC (version 0.11.5)