FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27691447
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN313290.11313601633024088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG64600.051.50879745
CGTTTCG95550.050.62569433
GATCGGA97200.049.4255871
TATGCCG68850.049.3966448
CGTATGC69300.048.820346
CTCGTAT70750.047.0785444
ATCGGAA111550.044.492842
ACACGTC113850.043.76110513
AGTCACG112850.043.5333828
TTTCGGA112700.042.89529435
ACGTCTG119300.041.5291315
GTTTCGG117850.041.19563734
ACGTTTC119800.040.7241832
GTCACGT121050.040.584429
CGGAATC116650.039.6904838
TTCGGAA123100.039.55521836
TCGGAAT123050.039.09366637
AATCGCG12550.038.22627341
CACGTCT131300.037.8923914
ATGCCGT88750.037.53802549
Produced by FastQC (version 0.11.5)