FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27087720
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN297590.1098615904180935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG33750.030.28236243
CGTATGC36650.027.50767544
TATGCCG36050.027.48526846
CTCGTAT38050.026.58476842
TATCTCG46350.021.97136539
GATCGGA44100.021.7569071
CGCCAAT54850.019.63876733
TCTCGTA53200.019.40528741
ACACGTC55750.019.13198513
GTCACGC54150.018.99134829
ATCGGAA53600.018.675062
AGTCACG58350.018.10069728
ATCTCGT57700.018.07173340
ATGCCGT56350.018.01632547
CGCGCTA22500.016.68789746
ACGCCAA66150.016.59840432
ACGTCTG64900.015.95278515
GCCGTCT66800.014.73086349
CACGTCT76500.014.03342214
TGCCGTC76150.013.60651548
Produced by FastQC (version 0.11.5)