FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12525838
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2208021.7627722791880271TruSeq Adapter, Index 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1303311.0404972505631958No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG596550.4762555607057987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG320750.076.0673370
GATCGGA252500.066.214411
TCGTATG252300.065.7984443
CTCGTAT253200.064.9317642
TATGCCG256500.064.8216946
ATCGGAA259450.064.8016742
CGTATGC256750.064.6858944
ACACGTC261700.064.039413
ACGTCTG265150.063.062715
TCTCGTA262350.062.49331741
CACGATC261200.062.4160436
TCACGAT265350.061.9486335
ACGATCT264000.061.88724537
ATGCCGT268900.061.83401547
ATCTCGT265450.061.77510540
CACGTCT271450.061.72691314
TCGGAAG274200.061.467743
ATCACGA269150.061.30603434
CATCACG270850.061.0989833
CGTCTGA274200.060.9686216
Produced by FastQC (version 0.11.5)