FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24896202
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34350.036.73314343
TATGCCG38350.033.9915646
CGTATGC38850.033.01576244
CTCGTAT38800.031.80297942
GATCGGA41550.029.2911111
ATCGGAA48800.026.8568822
TCTCGTA48450.025.61166241
ACACGTC55050.024.17657713
ATCTCGT52650.023.43596640
ATGCCGT56000.023.40305347
ACGTCTG65150.020.53519215
CACGTCT71900.018.22076814
CGTCTGA77000.017.23948516
TCGGAAG78700.017.227153
GCCGTCT75500.017.03734449
CCGTCTT75600.016.83133950
TGCCGTC79500.016.39814448
CACACGT82850.015.9383912
CATCTCG78950.015.80467539
GCACACG94150.015.35420111
Produced by FastQC (version 0.11.5)