FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8547150
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA855901.0013864270546322TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG709770.8304171565960583No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157110.18381565785086257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA96100.063.4171071
TCGTATG97550.063.36022645
CTCGTAT97900.062.63585744
TATGCCG98700.062.62492448
CGTATGC98900.062.46056446
ATCGGAA100350.061.5457762
AACGATC101150.060.3041338
AGTCACG102850.060.13493328
TCTCGTA102100.060.022443
ACACGTC104150.059.98484413
GAAACGA103000.059.89307836
TGAAACG104650.059.04734435
AAACGAT104600.058.84609237
ATCTCGT104200.058.64421542
ACGATCT104250.058.54562839
ACGTCTG106950.058.41509615
ATGCCGT106850.057.91462349
GTCACGT107150.057.88347629
CACGTCT108250.057.74601714
CGTGAAA108050.057.2489433
Produced by FastQC (version 0.11.5)