FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24972406
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1550440.620861281848453TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG192800.062.40442743
GATCGGA195900.061.861281
CGTATGC197150.061.2408344
TATGCCG198500.061.0549446
CTCGTAT195500.060.74020842
ACGGCTA205600.059.38332432
ATCGGAA209950.059.0464972
ACACGTC212050.058.32559213
TCTCGTA203300.057.6047641
AGTCACG213050.057.4492328
ATGCCGT215550.056.0664547
ATCTCGT208000.055.75016440
ACGTCTG222950.055.42751715
CGGCTAC221600.054.90916433
TCACGGC228000.053.5307130
CGTCTGA230150.053.49702516
GTCACGG228950.053.33798629
TCGGAAG233750.053.0919533
CACGTCT234250.052.8129814
GCCGTCT228850.052.5837649
Produced by FastQC (version 0.11.5)