FastQCFastQC Report
Wed 15 May 2024
EGAF00004684215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76452262
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4666510.6103822016410712No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1376210.1800090623871927Illumina RNA PCR Primer (100% over 45bp)
TCCGCCAGTACTTTACCGTCTTCGACAGGGCAAACAACCAGGTCGGCCTG1309390.17126896781680573No Hit
CGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT890660.1164988421140502Illumina RNA PCR Primer (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)