FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44053094
Sequences flagged as poor quality0
Sequence length60-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2700910.6131033611396285No Hit
GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCC645140.14644601353085437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTCG118900.068.3104670
GGTCCGC777550.055.88717770
GATAGCG88350.046.7303246
TATAGCG34700.045.1305436
ACCGAGT116650.043.3271938
CGATAGC73150.040.495
TGCAGAC211700.040.11511670
TAACGCA71900.039.22776840
CGGTACA72400.038.9119821
AGCGCCT154450.036.8339770
GCGGTAC77050.036.83304220
TACCGGC52950.036.73246813
CTGAATG623750.036.0846026
CGAACCA77250.035.8366817
CCCGTCC214650.035.7032570
CGAATAG32050.035.64234
GACTACG106100.035.61772515
CGATAGT55200.035.1777327
GTAACGC80550.035.1020739
GAATGTC379050.034.7139368
Produced by FastQC (version 0.11.5)