FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40147509
Sequences flagged as poor quality0
Sequence length61-76
%GC21

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACT652250.068.244447
CGTACTG655100.067.206278
TAGGAGC828050.067.062988
GGAGCTT872350.067.0564510
GTAAGAT957050.067.026849
GTACTGT658550.066.8650369
ACGCATT418950.066.8617210
AATCGGC325300.066.208427
AGCACTC590500.066.2005167
GGTAAGA967350.066.183098
CGGCATT323850.066.16994510
TCGTCCT343700.066.165587
GGCACTT414250.066.0794459
ATCGGCA325150.066.044788
GCTTCCC442400.066.023217
AACACGC431150.065.949757
CGTGATT243050.065.8782969
TCTGCTA500100.065.868358
GACGTAC153900.065.8126
GCACTCT592000.065.7960058
Produced by FastQC (version 0.11.5)