FastQCFastQC Report
Mon 6 May 2024
EGAF00004684244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68699391
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5198970.7567709006328747No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2318040.3374178382454657No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATATCGTATGC1082310.1575428812753231RNA PCR Primer, Index 13 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC846210.12317576439651408RNA PCR Primer, Index 13 (100% over 50bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATATCGTATG833210.12128346232355976RNA PCR Primer, Index 13 (97% over 49bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA761620.11086270036949819No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)