FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004684244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68699391
Sequences flagged as poor quality0
Sequence length35-76
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5131210.746907639981845No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATATCGTATGC1048160.15257194929137RNA PCR Primer, Index 13 (98% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA971860.14146559174010728No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC828550.12060514481125459RNA PCR Primer, Index 13 (100% over 50bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATATCGTATG713740.10389320627310947RNA PCR Primer, Index 13 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)