FastQCFastQC Report
Wed 15 May 2024
EGAF00004684245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68699391
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCCTACCAATAACGTAGAATAAAAACATAACCCACTGAAAAAAAAAA1771420.25785090292867374No Hit
GCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG1705610.24827148758858722No Hit
GCCCACTTTAGATGTATCTAATTCTCCTGACTGTTCTTCCCAGGGGAGTG1585020.230718202436467No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1524780.2219495657537925Illumina RNA PCR Primer (100% over 45bp)
TCCGCCAGTACTTTACCGTCTTCGACAGGGCAAACAACCAGGTCGGCCTG1495090.21762783894256066No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1448710.21087668739305127No Hit
TGTCCTACCAATAACGTAGAATAAAAACATAACCCACTGAAAAAAAAAAA1383520.2013875203056749No Hit
CTGTCCTACCAATAACGTAGAATAAAAACATAACCCACTAAAAAAAAAAA1038890.15122259235165564No Hit
CGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT875710.1274698344851412Illumina RNA PCR Primer (100% over 44bp)
GTGCCCACTTTAGATGTATCTAATTCTCCTGACTGTTCTTCCCAGGGGAG867950.1263402757092854No Hit
TGTCCTACCAATAACGTAGAATAAAAACATAACCCACTAAAAAAAAAAAA848410.12349600013193712No Hit
GCTTCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG705820.10274035762558652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)