FastQCFastQC Report
Wed 27 Sep 2023
EGAF00004684245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68699391
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG1704660.24813320397556365No Hit
GCCCACTTTAGATGTATCTAATTCTCCTGACTGTTCTTCCCAGGGGAGTG1547280.22522470395698269No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1517260.22085494178543738Illumina RNA PCR Primer (100% over 45bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1393450.20283294796601617No Hit
TCCGCCAGTACTTTACCGTCTTCGACAGGGCAAACAACCAGGTCGGCCTG1277140.18590266688099172No Hit
CGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT871300.12682790739731595Illumina RNA PCR Primer (100% over 44bp)
GTGCCCACTTTAGATGTATCTAATTCTCCTGACTGTTCTTCCCAGGGGAG794290.11561820104053033No Hit
GCTTCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG705330.10266903239360595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)