FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19590518
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG423700.21627809943565554TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA58200.039.956221
CTCGTAT54400.039.7333844
TCTCGTA55050.039.38841643
ATCGGAA60400.038.63772
TCGTATG51100.037.2521645
ACACGTC62900.036.90107313
CGTCTGA63200.036.47680316
ATCTCGT61100.035.85986742
TCGGAAG65650.035.5821383
ACGTCTG67900.034.15064215
CGGAAGA69650.033.4417274
CACGTCT70500.032.92308814
AATCTCG70850.030.98994641
CACACGT75850.030.95671712
GCACACG76250.030.67633411
ACATGTC88350.025.62920832
TCTGAAC99700.023.43745418
AGAGCAC100950.023.4145228
GTCTGAA102150.022.94242717
GAATCTC98450.022.87740940
Produced by FastQC (version 0.11.5)