FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19089642
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT20150.031.4832742
CGTATGC21650.030.752544
TCGTATG21950.029.5134143
TCTCGTA23050.027.8162841
ATCGGAA24650.027.2051982
CGTCTGA26350.025.86402516
GATCGGA26000.025.74251
ACACGTC27400.024.5445313
TCGGAAG28400.023.8506773
ACGTCTG29800.022.64326915
CACGTCT33000.020.65203314
CGGAAGA33700.020.099684
GTATGCC32800.019.8024145
CACACGT36300.018.77457412
ATCTCGT37000.017.81745340
GCACACG40650.016.76548811
GTCACAC45750.015.10277629
CCAGTCA59000.012.58601826
AGAGCAC57550.012.193098
CAGTCAC61200.011.76638727
Produced by FastQC (version 0.11.5)