FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33300089
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG3053510.9169675192159397TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA346950.043.446761
ATCGGAA349850.043.146392
TCGTATG319500.043.0935645
CTCGTAT318600.043.07192244
TCTCGTA319850.042.89713743
TATCTCG323500.042.48466541
GTCACGA327700.042.44151329
ACACGTC356100.042.20766413
ACGAGTG330400.042.0695432
CACGAGT331050.041.9665531
ATCTCGT327500.041.96198742
CGTCTGA357650.041.9022316
TCGGAAG361850.041.752693
AGTCACG337300.041.43168328
CGGAAGA367050.041.1734434
ACGTCTG365650.041.052715
CACGTCT370600.040.5562614
CACACGT375550.040.09357512
CGAGTGG348150.039.91839233
GGATATC350300.039.31144738
Produced by FastQC (version 0.11.5)