FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590928
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGAGTCCTCGACTCTCAGGCTGCTCATTTCCAGATATA30150.5102144423686135No Hit
ACCATATACTACGCAGACTCTGTTAAGGGCCGATTTATCATCTCCAAAGA24350.41206373703733784No Hit
GTATATGGTAGAACCACTTTTACTGATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT20320.3438659193675033No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18990.3213589472829177No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16900.2859908482928546No Hit
GTTTATTACTGTGCGAGAGACTGGGTGCAGTGGCTGGTCCCCGATGCTTT16470.27871415807001865No Hit
GATCTATGCTGCACACATTTTGCAAAGTGGGGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCA15230.257730214171608No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14200.24030000270760565No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13490.22828500257222537No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13060.22100831234938942No Hit
GACTTATAGAATCCCTCAGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12600.21322394606449516No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11880.20103972057509545No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11690.19782443884872608No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11630.19680908672460945No Hit
CTATAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAATCTGTC11580.19596295995451224No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATG11170.18902472043971516No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10930.18496331194324858No Hit
ATCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTC10890.18428641052717082No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10860.1837787344651125No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTACTATTTTTGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT10860.1837787344651125No Hit
GTCCTGATCTATGCTGCACACATTTTGCAAAGTGGGGTCCCATCAAGGTT10790.18259415698697642No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10780.18242493163295698No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10660.1803942273847237No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10360.17531746676414048No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10290.17413288928600437No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9990.16905612866542116No Hit
CTCCTGTGCAGCCTCTGGATTCAGCCTCAATAGTTATGAAATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG9910.16770232583326564No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9790.16567162158503237No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC9750.16499472016895458No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9680.1638101426908185No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9560.16177943844258522No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9530.1612717623805269No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9480.1604256356104297No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9400.1590718327782742No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9400.1590718327782742No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9120.15433352286572985No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9000.15230281861749656No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8910.1507797904313216No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8750.14807218476701053No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8520.1441800016245634No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8520.1441800016245634No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8460.14316464950044674No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8440.14282619879240788No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8440.14282619879240788No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8430.14265697343838843No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATGTAAAT8270.13994936777407738No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC8150.13791866352584412No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT8020.13571873392359135No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7980.13504183250751362No Hit
GTGCTGGGTTTTCCTTGTTACTATTTTTGAAGGTGTCCAGTGTGAGGTGC7950.1345341564454553No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7840.13267267755124143No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT7770.13148810007310535No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTAAACCTTGATGGGACCCCA7770.13148810007310535No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTGAGAGCATTAGTAGTTATTTACATTGGTATCAGAAGAAA7750.13114964936506648No Hit
GATTTCACTCTTACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTATAGAATC7650.12945739582487206No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7630.12911894511683317No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC7570.12810359299271654No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7490.126749790160561No Hit
ACTTACTACTGTCAACAGACTTATAGAATCCCTCAGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA7360.12454986055830829No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGACATCAAAAGCATCGGGGA7300.12353450843419164No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7280.12319605772615277No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7230.12234993095605556No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7000.11845774781360843No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC6940.11744239568949179No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC6890.11659626891939458No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGA6870.1162578182113557No Hit
CCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATGTAAATAACTACTA6840.11575014214929738No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6840.11575014214929738No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT6810.11524246608723906No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6780.11473479002518074No Hit
CCATTGTCCCTTGGCCCCAGACATCAAAAGCATCGGGGACCAGCCACTGCACCCAGTCTCTCGCACAGTAATAAA6720.11371943790106408No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG6710.11355021254704466No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6490.10982725475861695No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6450.1091503533425392No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6380.1079657758644031No Hit
GAGTACGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATCAGCACTGAACA6380.1079657758644031No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6360.10762732515636422No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC6280.10627352232420871No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6250.10576584626215038No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6210.10508894484607262No Hit
GCCTGAGAGTCGAGGACTCGGCTGTTTATTACTGTGCGAGAGACTGGGTG6170.10441204342999486No Hit
GTCTCCGTCCTCCTTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT6150.10407359272195597No Hit
GCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATCAGCACTGAAC6080.1028890152438199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTA200.0032185163.76969
GTCGTAT501.1332304E-955.093139
TGTCGTA453.102832E-853.49413338
TTATACA2300.053.343612
TCGTATG2300.052.4386840
GAGCGAT2300.052.09547832
CTTATAC2300.051.888191
GTATTGA200.007706248751.1469271
GGTTACC200.007721736651.120962
ATGCCGT2450.050.92659444
CGAGACA2450.050.1031123
GCGATCT1850.050.01865434
CGTATGC2500.049.68573841
ACGAGAC2500.049.1010522
TATACAC2500.049.0761223
ATCTCGT1850.048.28526337
ATCTCCG2550.048.11791610
TACACAT2550.048.1179165
ATGTCGT507.168455E-848.09954537
TTTGGAG8950.047.79187870
Produced by FastQC (version 0.11.5)