FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590928
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGAGTCCTCGACTCTCAGGCTGCTCATTTCCAGATATA26020.4403243711585844No Hit
ACCATATACTACGCAGACTCTGTTAAGGGCCGATTTATCATCTCCAAAGA24570.4157866948257656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19820.3354046516665313No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19260.3259280318414426No Hit
GTATATGGTAGAACCACTTTTACTGATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT18080.3059594400671486No Hit
GTTTATTACTGTGCGAGAGACTGGGTGCAGTGGCTGGTCCCCGATGCTTT17100.28937535537324344No Hit
GATCTATGCTGCACACATTTTGCAAAGTGGGGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCA15180.2568840874015108No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14450.24453063655809165No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13960.2362385942111391No Hit
GACTTATAGAATCCCTCAGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12750.21576232637478676No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12660.2142392981886118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12380.20950098827606745No Hit
GTCCTGATCTATGCTGCACACATTTTGCAAAGTGGGGTCCCATCAAGGTT11680.19765521349470663No Hit
CTATAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAATCTGTC11440.19359380499824005No Hit
CTCCTGTGCAGCCTCTGGATTCAGCCTCAATAGTTATGAAATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG11180.1891939457937346No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10830.1832710584030542No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTACTATTTTTGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT10600.17937887526060706No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10380.17565591747217935No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10380.17565591747217935No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10060.17024070614355724No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10060.17024070614355724No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATG9960.16854845260336285No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9480.1604256356104297No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9470.16025641025641024No Hit
ATCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTC9430.15957950884033248No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9250.15653345246798256No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9180.15534887498984648No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9170.15517964963582703No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9170.15517964963582703No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9080.15365662144965206No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9000.15230281861749656No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9000.15230281861749656No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC8990.1521335932634771No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8960.1516259172014188No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8910.1507797904313216No Hit
GTGCTGGGTTTTCCTTGTTACTATTTTTGAAGGTGTCCAGTGTGAGGTGC8800.14891831153710774No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8530.14434922697858285No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8430.14265697343838843No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8290.14028781848211627No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8280.14011859312809682No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8220.13910324100398017No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8020.13571873392359135No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTGAGAGCATTAGTAGTTATTTACATTGGTATCAGAAGAAA8000.13538028321555248No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATGTAAAT7920.13402648038339696No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7830.13250345219722198No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTAAACCTTGATGGGACCCCA7830.13250345219722198No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC7810.13216500148918311No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC7700.13030352259496927No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7680.12996507188693038No Hit
ACTTACTACTGTCAACAGACTTATAGAATCCCTCAGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA7570.12810359299271654No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT7550.12776514228467767No Hit
GATTTCACTCTTACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTATAGAATC7540.12759591693065822No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7490.126749790160561No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC7450.12607288874448325No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7380.12488831126634718No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7260.12285760701811388No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7210.12201148024801667No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG7180.12150380418595837No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGACATCAAAAGCATCGGGGA7130.12065767741586116No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7110.12031922670782226No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6950.11761162104351124No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC6800.11507324073321962No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC6660.11270408577694746No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6580.11135028294479192No Hit
CCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATGTAAATAACTACTA6490.10982725475861695No Hit
GAGTACGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATCAGCACTGAACA6330.1071196490943059No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGA6280.10627352232420871No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6270.10610429697018926No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG6260.10593507161616983No Hit
GTGGGGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCACT6220.10525817020009207No Hit
CCATTGTCCCTTGGCCCCAGACATCAAAAGCATCGGGGACCAGCCACTGCACCCAGTCTCTCGCACAGTAATAAA6190.10475049413803372No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATTTCATAACTAT6160.10424281807597541No Hit
CTGTTAAGGGCCGATTTATCATCTCCAAAGACAACGCCAATAACTCACTA6010.1017044377656838No Hit
GTCTCCGTCCTCCTTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT6000.10153521241166436No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5960.10085831099558659No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC5960.10085831099558659No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC5940.10051986028754772No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT5910.1000121842254894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTTA200.007047555452.3158248
GTGGTAT950.050.239951
TTTGGAG8100.049.7761770
TATCTTT350.001250681940.02963650
GACAGGT1400.038.9615941
GTATCAA11300.038.9184951
CTTTGGA8700.034.52050869
TATCAAC12850.034.195082
GAGTTTA1900.034.0913931
AATGGTA1551.0356962E-733.03119370
ATCAACG13350.032.90883
TATCTGG8400.032.76084570
TCAACGC13600.032.3038564
GCCGAGT7700.032.29032511
CAACGCA13650.032.1855285
AACGCAG13700.032.0707746
TTCTTAA800.003749997131.99896670
TCGACTC7850.031.6947720
ACAATGG7100.031.54827570
CTCGACT7900.031.48617219
Produced by FastQC (version 0.11.5)