FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739002
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27720.3751004733410735No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23800.32205596195950753No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18700.2530439701110416No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18500.2503376174895332No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16260.22002646812863835No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16090.21772606840035616No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15420.2086597871183028No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15200.2056827992346435No Hit
GACTTATACTACTTTCTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCAAACGAACTGTGGCTGCACCAT15120.20460025818604008No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13690.18524983694225455No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCGACTATTTACATTGGTATCAACACAGA13420.18159626090321812No Hit
GTATCAACACAGACCAGGGAAACCCCCTGCGCTCCTGATCTATGCTGCAT13420.18159626090321812No Hit
GTCTTCAGGCTGCAGACTGTTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCACTGAACCTCGATGGGA13020.1761835556602012No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12940.1751010146115978No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12780.17293593251439102No Hit
GATCTATGCTGCATCCACTTTGCAGAATGGAGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGCACAGATTTCA12770.1728006148833156No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12690.17171807383471221No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12660.17131212094148596No Hit
GACCAGGGAAACCCCCTGCGCTCCTGATCTATGCTGCATCCACTTTGCAG12390.16765854490244952No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11760.15913353414469786No Hit
GTATAAGTCTGTAGACAGAAGTATGTCGCAAAGTCTTCAGGCTGCAGACTGTTGATGGTGAGAGTGAAATCTGTG11750.15899821651362245No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAGAATGGAGTCCCATCGAGGTT11710.15845694598932072No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11410.15439741705705803No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11270.1525029702220021No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11260.15236765259092666No Hit
GTGTTGATACCAATGTAAATAGTCGCTAATGGTCTGACTTGCCCGGCAAG11160.15101447628017245No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCAGCTTGGTCCCCTGGCCAAAAGTG11070.14979661760049365No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCGA10990.14871407655189026No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10590.14330137130887333No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCGGATGTGACAT10530.14248946552242076No Hit
CTGCTACTCTGGCTCCGAGGTGCCGGATGTGACATCCGGATGACCCAGTC10130.13707676027940383No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT9920.13423509002681996No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9740.13179937266746233No Hit
GATTTCACTCTCACCATCAACAGTCTGCAGCCTGAAGACTTTGCGACATA9510.1286870671527276No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9440.12773984373519964No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9270.12543944400691742No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAAGATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC9170.12408626769616321No Hit
CTGTTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCACTGAACCT8900.12043269165712678No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8810.11921483297744798No Hit
GTTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCACTGAACCTCG8770.11867356245314628No Hit
ACACAGACCAGGGAAACCCCCTGCGCTCCTGATCTATGCTGCATCCACTTTGCAGAATGGAGTCCCATCGAGGTT8680.11745570377346747No Hit
CTTATACTACTTTCTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCAAA8630.11677911561809033No Hit
CCATCAACAGTCTGCAGCCTGAAGACTTTGCGACATACTTCTGTCTACAG8590.11623784509378866No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8560.11583189220056238No Hit
GATCAGGAGCGCAGGGGGTTTCCCTGGTCTGTGTTGATACCAATGTAAAT8480.114749351151959No Hit
CACCATCAACAGTCTGCAGCCTGAAGACTTTGCGACATACTTCTGTCTAC8410.11380212773443105No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8270.1119076808993751No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8270.1119076808993751No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8240.11150172800614884No Hit
GAAATATAGGGCCGTGTCTGCGGCGGTCACAGACCTCAGCCTCAGGGAGA8220.11123109274399799No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8110.10974259880216833No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCGGATGTGACATCCGGATGACCCAGTCTCCATCCTCC8050.10893069301571578No Hit
GGGAAACCCCCTGCGCTCCTGATCTATGCTGCATCCACTTTGCAGAATGG7980.10798346959818783No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7710.1043298935591514No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7690.10405925829700055No Hit
GAAGTATGTCGCAAAGTCTTCAGGCTGCAGACTGTTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCA7670.1037886230348497No Hit
GCCTGAAGACTTTGCGACATACTTCTGTCTACAGACTTATACTACTTTCTACACTTTTGGCCAGGGGACCAAG7650.10351798777269886No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7630.10324735251054801No Hit
CTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGAAAGTAGTATAAGTCTGTA7630.10324735251054801No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA7620.1031120348794726No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGAGCAAACGAACTGTGGCTGCACCA7610.10297671724839716No Hit
GAGTGAAATCTGTGCCAGATCCACTGCCACTGAACCTCGATGGGACTCCA7560.10230012909302004No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7420.10040568225796412No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7410.1002703646268887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)