FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739002
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27910.37767150833150653No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20900.2828138489476348No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19850.2686054976847153No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17630.23856498358597136No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16200.21921456234218578No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15900.21515503340992312No Hit
GACTTATACTACTTTCTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCAAACGAACTGTGGCTGCACCAT15610.21123082210873584No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15140.20487089344819095No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14910.2017585879334562No Hit
GTCTTCAGGCTGCAGACTGTTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCACTGAACCTCGATGGGA13640.18457324878687745No Hit
GATCTATGCTGCATCCACTTTGCAGAATGGAGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGCACAGATTTCA13140.17780736723310628No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12560.16995894463073172No Hit
GTATCAACACAGACCAGGGAAACCCCCTGCGCTCCTGATCTATGCTGCAT12510.1692823564753546No Hit
GACCAGGGAAACCCCCTGCGCTCCTGATCTATGCTGCATCCACTTTGCAG12430.1681998154267512No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAGAATGGAGTCCCATCGAGGTT12380.1675232272713741No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11830.1600807575622258No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11810.15981012230007496No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCGACTATTTACATTGGTATCAACACAGA11750.15899821651362245No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11710.15845694598932072No Hit
GTGTTGATACCAATGTAAATAGTCGCTAATGGTCTGACTTGCCCGGCAAG11410.15439741705705803No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11200.15155574680447415No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11000.1488493941829657No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10820.14641367682360806No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCGA10770.14573708866823093No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10720.14506050051285382No Hit
CTGCTACTCTGGCTCCGAGGTGCCGGATGTGACATCCGGATGACCCAGTC10710.1449251828817784No Hit
GTATAAGTCTGTAGACAGAAGTATGTCGCAAAGTCTTCAGGCTGCAGACTGTTGATGGTGAGAGTGAAATCTGTG10690.14465454761962757No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10600.14343668893994876No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCAGCTTGGTCCCCTGGCCAAAAGTG10590.14330137130887333No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10380.14045970105628944No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9940.1345057252889708No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9740.13179937266746233No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9660.13071683161885894No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9640.1304461963567081No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCGGATGTGACAT9550.1292283376770293No Hit
GATTTCACTCTCACCATCAACAGTCTGCAGCCTGAAGACTTTGCGACATA9510.1286870671527276No Hit
CTGTTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCACTGAACCT9490.12841643189057675No Hit
ACACAGACCAGGGAAACCCCCTGCGCTCCTGATCTATGCTGCATCCACTTTGCAGAATGGAGTCCCATCGAGGTT9100.12313904427863524No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9080.1228684090164844No Hit
CACCATCAACAGTCTGCAGCCTGAAGACTTTGCGACATACTTCTGTCTAC9040.1223271384921827No Hit
GAAATATAGGGCCGTGTCTGCGGCGGTCACAGACCTCAGCCTCAGGGAGA8830.11948546823959882No Hit
CTTATACTACTTTCTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCAAA8730.11813229192884457No Hit
GTTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCACTGAACCTCG8700.11772633903561831No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAAGATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC8640.11691443324916577No Hit
CCATCAACAGTCTGCAGCCTGAAGACTTTGCGACATACTTCTGTCTACAG8540.11556125693841153No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8360.11312553957905391No Hit
GGGAAACCCCCTGCGCTCCTGATCTATGCTGCATCCACTTTGCAGAATGG8060.10906601064679122No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8060.10906601064679122No Hit
GCCTGAAGACTTTGCGACATACTTCTGTCTACAGACTTATACTACTTTCTACACTTTTGGCCAGGGGACCAAG8010.10838942249141409No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7780.10527711697667935No Hit
GATCAGGAGCGCAGGGGGTTTCCCTGGTCTGTGTTGATACCAATGTAAAT7750.1048711640834531No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7670.1037886230348497No Hit
CTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGAAAGTAGTATAAGTCTGTA7660.10365330540377429No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7490.10135290567549209No Hit
GAAGTATGTCGCAAAGTCTTCAGGCTGCAGACTGTTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCA7430.10054099988903954No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7430.10054099988903954No Hit
TCCCTGGTCTGTGTTGATACCAATGTAAATAGTCGCTAATGGTCTGACTTGCCCGGCAAGTGATGGTGACT7410.1002703646268887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)