FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927360
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37910.4087948585231194No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33500.36124051069703245No Hit
TCTATGGTGTGACCACTTTGCAAAGTGGGGTCCCATCAAGGTTCCGTGGCGGTGGCTCTGGGACAGAGTTCACT27040.2915804002760525No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22300.24046756383712906No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22120.23852657004830918No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21980.23701690821256038No Hit
GAGTTCACTCTCACCATCAGTAGTCTGCAACCTGAAGATTTTGCAACTTACTTTTGTCAGCAGAGCTTCACTACC21430.23108609385783296No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21050.2269884403036577No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTACCAATTTTTTAAGTTGGTATCAGCACAAA20910.2254787784679089No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT20260.21846963423050378No Hit
TCACTACCCCTCTCACCTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGA19400.2091959972394755No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19380.20898033126293997No Hit
CATCTATGGTGTGACCACTTTGCAAAGTGGGGTCCCATCAAGGTTCCGTGGCGGTGGCTCTGGGACAGAGTTCA18950.2043435127674258No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18490.19938319530710835No Hit
GGGTAGTGAAGCTCTGCTGACAAAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTACTGATGGTGAGAGTGAACT18190.1961482056590752No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18180.19604037267080746No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18020.19431504485852313No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17890.1929132160110421No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17880.19280538302277433No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17700.19086438923395446No Hit
CACCATAGATGAGGACTTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAACTTAAAAAATTGGTAATGGTCTGA17040.1837474120082816No Hit
GTCTCCGGTGGCTCCATCGGCAGTTCTTATTGGAGCTGGATCCGGCAGTC16850.18169858523119392No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCC16610.17911059351276742No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16200.17468944099378883No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15730.16962129054520358No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAGGTG15720.16951345755693584No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15570.16789596273291926No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15420.1662784679089027No Hit
GTCCCAGAGCCACCGCCACGGAACCTTGATGGGACCCCACTTTGCAAAGT15410.1661706349206349No Hit
CCATAGATGAGGACTTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAACT14990.16164164941338854No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14730.1588379917184265No Hit
CTCTGGGACAGAGTTCACTCTCACCATCAGTAGTCTGCAACCTGAAGATTTTGCAACTTACTTTTGTCAGCAGAG14720.15873015873015872No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14490.15625No Hit
GAGTGAACTCTGTCCCAGAGCCACCGCCACGGAACCTTGATGGGACCCCA14390.15517167011732227No Hit
ATAATAGACGGCCGTGTCCGCAGCGCTCACAGACATCAACTTCAGGGAGA14270.15387767425810903No Hit
CTTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAACTTAAAAAATTGGTA14250.1536620082815735No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14010.15107401656314698No Hit
CTACTGATGGTGAGAGTGAACTCTGTCCCAGAGCCACCGCCACGGAACCT13320.14363354037267081No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13310.14352570738440304No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13240.14277087646652864No Hit
GTCCTGGACCTCCTGTGCACGAACATGAAGCATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13220.14255521048999312No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12780.13781055900621117No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12670.1366243961352657No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12650.13640873015873015No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12520.13500690131124912No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12360.1332815734989648No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12250.13209541062801933No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12130.13080141476880608No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12100.13047791580400275No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11990.12929175293305728No Hit
CCCCTAAAGTCCTCATCTATGGTGTGACCACTTTGCAAAGTGGGGTCCCA11760.12681159420289853No Hit
AACCAGGGAAAGCCCCTAAAGTCCTCATCTATGGTGTGACCACTTTGCAA11590.12497843340234643No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11520.12422360248447205No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11460.12357660455486541No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11430.12325310559006211No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11400.1229296066252588No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11270.12152777777777778No Hit
AGTCAGACCATTACCAATTTTTTAAGTTGGTATCAGCACAAACCAGGGAA11240.12120427881297446No Hit
CAATAATAGACGGCCGTGTCCGCAGCGCTCACAGACATCAACTTCAGGGAGAACTGGTTCTTAGACGTGTCTA10870.11721445824706696No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10830.11678312629399587No Hit
GCACAAACCAGGGAAAGCCCCTAAAGTCCTCATCTATGGTGTGACCACTTTGCAAAGTGGGGTCCCATCAAGGTT10740.1158126293995859No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10710.11548913043478261No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10650.11484213250517597No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10580.1140873015873016No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10450.11268547273982057No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10190.10988181504485853No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9970.10750948930296755No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9970.10750948930296755No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9680.10438233264320222No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA9580.1033040027605245No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT9550.10298050379572117No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9540.10287267080745341No Hit
CACGTGCACTGTCTCCGGTGGCTCCATCGGCAGTTCTTATTGGAGCTGGATCCGGCAGTCCCCAGGAAAGGGATT9530.10276483781918565No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9490.10233350586611456No Hit
GACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT9460.10201000690131123No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT9450.10190217391304347No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9450.10190217391304347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGT200.0075527251.41026727
TCGTATG1750.049.35654440
ATCTCGT1600.047.36646737
CGTATGC1850.046.7267741
GTGGTAT1500.045.670891
GTATTAG950.043.2671621
GATCTCG1800.042.07840736
CACGAGA2050.041.7451321
CTTATAC2150.041.4224361
GTATGCC2100.041.1887442
ACGAGAC2100.040.74922
GACTAGG2200.040.49194326
TACACAT2200.040.4526445
TATACAC2200.040.4504663
ATGCCGT2150.040.31215344
CGAGACT2150.039.8056423
AGACTAG2250.039.57503525
ATCTCCG2200.038.89677410
CCGTCTT2350.038.48689747
ACTAGGC2150.038.25880427
Produced by FastQC (version 0.11.5)