FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927360
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36400.392512077294686No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28680.30926501035196685No Hit
TCTATGGTGTGACCACTTTGCAAAGTGGGGTCCCATCAAGGTTCCGTGGCGGTGGCTCTGGGACAGAGTTCACT26060.2810127674258109No Hit
GAGTTCACTCTCACCATCAGTAGTCTGCAACCTGAAGATTTTGCAACTTACTTTTGTCAGCAGAGCTTCACTACC22120.23852657004830918No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22090.23820307108350586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22070.23798740510697033No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20840.2247239475500345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20540.22148895790200135No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTACCAATTTTTTAAGTTGGTATCAGCACAAA20500.2210576259489303No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20130.21706780538302278No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19360.20876466528640444No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18600.20056935817805385No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18070.19485420979986198No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17270.18622757073844032No Hit
CATCTATGGTGTGACCACTTTGCAAAGTGGGGTCCCATCAAGGTTCCGTGGCGGTGGCTCTGGGACAGAGTTCA17240.185904071773637No Hit
TCACTACCCCTCTCACCTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGA17210.1855805728088337No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16540.17835576259489302No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16440.1772774327122153No Hit
GGGTAGTGAAGCTCTGCTGACAAAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTACTGATGGTGAGAGTGAACT16290.17565993788819875No Hit
GTCTCCGGTGGCTCCATCGGCAGTTCTTATTGGAGCTGGATCCGGCAGTC16170.1743659420289855No Hit
CACCATAGATGAGGACTTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAACTTAAAAAATTGGTAATGGTCTGA16000.1725327812284334No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15780.17016045548654243No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAGGTG15770.17005262249827469No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15110.1629356452726018No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15090.16271997929606627No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15020.16196514837819184No Hit
CTCTGGGACAGAGTTCACTCTCACCATCAGTAGTCTGCAACCTGAAGATTTTGCAACTTACTTTTGTCAGCAGAG14960.16131815044858525No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14930.16099465148378192No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14090.15193668046928915No Hit
GTCCCAGAGCCACCGCCACGGAACCTTGATGGGACCCCACTTTGCAAAGT13940.1503191856452726No Hit
CCATAGATGAGGACTTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAACT13910.14999568668046928No Hit
CTTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAACTTAAAAAATTGGTA13890.14978002070393373No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13570.1463293650793651No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13510.14568236714975846No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13480.14535886818495514No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13380.14428053830227744No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13230.14266304347826086No Hit
CTACTGATGGTGAGAGTGAACTCTGTCCCAGAGCCACCGCCACGGAACCT13170.14201604554865424No Hit
GAGTGAACTCTGTCCCAGAGCCACCGCCACGGAACCTTGATGGGACCCCA13150.1418003795721187No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCC12760.13759489302967565No Hit
AGTCAGACCATTACCAATTTTTTAAGTTGGTATCAGCACAAACCAGGGAA12750.13748706004140787No Hit
ATAATAGACGGCCGTGTCCGCAGCGCTCACAGACATCAACTTCAGGGAGA12720.13716356107660455No Hit
GTCCTGGACCTCCTGTGCACGAACATGAAGCATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12720.13716356107660455No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12510.13489906832298137No Hit
CCCCTAAAGTCCTCATCTATGGTGTGACCACTTTGCAAAGTGGGGTCCCA12140.13090924775707383No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12040.12983091787439613No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12020.1296152518978606No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11800.12724292615596963No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11640.1255175983436853No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11600.12508626639061424No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11560.12465493443754314No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11450.12346877156659765No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11400.1229296066252588No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11380.12271394064872325No Hit
AACCAGGGAAAGCCCCTAAAGTCCTCATCTATGGTGTGACCACTTTGCAA11290.12174344375431331No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11160.12034161490683229No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10760.11602829537612147No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC10700.11538129744651485No Hit
GCACAAACCAGGGAAAGCCCCTAAAGTCCTCATCTATGGTGTGACCACTTTGCAAAGTGGGGTCCCATCAAGGTT10570.11397946859903381No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10500.11322463768115942No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10190.10988181504485853No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10000.10783298826777088No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9910.10686249137336094No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9820.10589199447895101No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9800.10567632850241546No Hit
GTGGGGTCCCATCAAGGTTCCGTGGCGGTGGCTCTGGGACAGAGTTCACT9740.10502933057280883No Hit
CAATAATAGACGGCCGTGTCCGCAGCGCTCACAGACATCAACTTCAGGGAGAACTGGTTCTTAGACGTGTCTA9740.10502933057280883No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA9410.10147084195997239No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9300.10028467908902693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)