FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499396
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC17310.3466187154082131No Hit
GATTATTACTGTTCAGTCTGGGATTACAGCCTGAGTGGTCGGGTTTTCGG15630.3129780775176413No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC15480.3099744491345546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13420.26872461934016295No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12660.2535062355325233No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11640.2330815625275333No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11530.2308789017132696No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT11180.2238704354860672No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11130.22286922602503825No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10750.2152600341212184No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10210.20444697194210606No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10070.2016435854512251No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAAAACCCGACCACTCAGGCTGTAATCCCAGA10020.20064237599019616No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9920.1986399570681383No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9750.19523584490063997No Hit
GTCTGGGATTACAGCCTGAGTGGTCGGGTTTTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGG9720.1946351192240226No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGAGGTCAC9440.18902834624226064No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC9390.18802713678123173No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9130.1828208475838813No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8960.179416735416383No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8950.1792164935241772No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGGTGTTCATTTCCAGATAGA8610.17240826918918054No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTC8590.17200778540476896No Hit
GACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAAAACCCGACCACTCAGG8260.16539980296197807No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC8170.163597625932126No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA8110.1623961745788913No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7780.1557881921361004No Hit
CTCTTGTTCTGGAAGCACCTCCAACATCGGACGTAATTCTGTATCTTGGTTCCGGCAACTCCCGGGAACGGCCCC7490.14998117726213264No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7420.14857948401669216No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7390.14797875834007482No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7240.14497512995698805No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7130.1427724691427244No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7120.14257222725051863No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG7080.1417712596816955No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC6950.13916811508302027No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6780.13576400291552196No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6770.13556376102331616No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT6750.1351632772389046No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAAAAC6670.1335613421012583No Hit
CCCCTGAGGGCCGCTGATTATTGTTAAAGATGAGGAGTCTGGGGGCCGTTCCCGGGAGTTGCCGGAACCAAGATA6410.12835505290390792No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6320.12655287587405586No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGGGAACCTATGAACATTCTGTAGGGGC6220.12455045695199801No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGGGAACCTATGAACATTC6200.12414997316758646No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6170.1235492474909691No Hit
CCTCCAACATCGGACGTAATTCTGTATCTTGGTTCCGGCAACTCCCGGGA6140.12294852181435173No Hit
GTCTGGGGGCCGTTCCCGGGAGTTGCCGGAACCAAGATACAGAATTACGT6100.1221475542455286No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG6090.12194731235332282No Hit
TTACTGTTCAGTCTGGGATTACAGCCTGAGTGGTCGGGTTTTCGGCGGAG6090.12194731235332282No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6050.12114634478449969No Hit
GTATATTACTGTGCGAGAGAGGGAGTGGGACCTTCCACCCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT5980.11974465153905918No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGGAGGTCACCATCTCTTGTTCTGG5980.11974465153905918No Hit
GCCCCCAGACTCCTCATCTTTAACAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT5880.11774223261700133No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT5770.11553957180273772No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC5620.11253594341965092No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT5530.11073376638979888No Hit
ATCCCAGACTGAACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC5350.10712941233009475No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5350.10712941233009475No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5080.10172288124053856No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5000.10012094610289231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)