FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117240
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36450.32625040277827505No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC36180.3238337331280656No Hit
CTGCTATTCCTCGCCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC35310.3160466864773907No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA32240.2885682574916759No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG31710.2838244244745981No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30050.2689663814399771No Hit
CCCTTAATATCTTCGGAACTGGGACCAGGGTCACCGTCCTAGGTCAGCCC29450.26359600443951164No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28350.2537503132719917No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG27650.24748487343811534No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC27470.2458737603379757No Hit
GTAGTAAACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGCTTCAGGGAGAACTGGTTCTTAGACGTGTCTAACGA26560.23772868855393647No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25040.22412373348609074No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT24780.22179657011922238No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT24740.22143854498585802No Hit
GACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGATATTAAGGGTGCTG24150.21615767426873367No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22220.19888296158390317No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22200.198703949017221No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC21610.19342307830009667No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTACAACTATGTCTCCTGGTACCAACAACA21340.19100640864988722No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTACAACTATGTCTCCTGGTACCAACAACACCCCGATAAGGC21200.18975332068311196No Hit
ACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGATATTAAGGGTGCTGC20840.1865310944828327No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20680.18509899394937523No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGATATTAAGGGTGCTGCTGCTTGTATATGAG20620.1845619562493287No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20400.18259281801582472No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGATATTAAGGGTG20190.1807131860656618No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19610.17552182163187857No Hit
ATATTAAGGGTGCTGCTGCTTGTATATGAGCTGCAGAAATAATCAGCCTC17910.16030575346389317No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17070.15278722566324157No Hit
GGCCTGGGCTCTGCTATTCCTCGCCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17050.1526082130965594No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT16840.15072858114639648No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16720.14965450574630337No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16320.14607425441265975No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15640.13998782714546562No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15420.13801868891196162No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACCCCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15230.13631806952848088No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14750.13202176792810857No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14550.13023164226128675No Hit
GACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14540.13014213597794566No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14320.12817299774444166No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13860.1240557087107515No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13200.11814829401023952No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12940.11582113064337117No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12860.11510508037664244No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12800.1145680426765959No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12620.11295692957645628No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12470.1116143353263399No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12360.11062976620958792No Hit
CAACTATGTCTCCTGGTACCAACAACACCCCGATAAGGCCCCCAAACTCATGATTTATGATGTCACTCATCGGCC12330.11036124735956464No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12290.11000322222620028No Hit
GCCCAGAGATGGTCAGGGAAGCCGTACTGCCAGACTTGGAGCCGGAGAAG12280.10991371594285919No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12220.10937667824281265No Hit
CTACTGGAACTGGATCCGCCAGTTCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCCATTACAGTGGGTCCAC12200.10919766567613046No Hit
GTTTACTACTGTGCGGGGAGATCTTGCAGTGGTGGTGCCTGCCTGAACTGGCTCGACCCCTGGGGCCAGGGAGCC11950.10696000859260321No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCGCCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT11930.106780996025921No Hit
GATTATTTCTGCAGCTCATATACAAGCAGCAGCACCCTTAATATCTTCGG11750.10516988292578139No Hit
GCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11740.1050803766424403No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11710.10481185779241703No Hit
GGCTGGAGCCCAGAGATGGTCAGGGAAGCCGTACTGCCAGACTTGGAGCC11490.10284271955891304No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11380.10185815044216104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4050.052.214170
GGTATCA9900.044.8377761
TGGTATC2700.042.969412
GTGGTAT2850.040.735211
GCTTACC702.39761E-839.0236471
CGTATGC3100.038.99695641
GTATCAA24600.038.726211
TCGTATG3150.038.31893540
CTAGCGC3250.036.80030428
ACTAGCG3250.036.79205727
GACTAGC3250.036.7805226
TCTCGTA3000.036.7048238
AGACTAG3350.035.66661525
CACGAGA3300.035.1599421
ATGCCGT3450.034.1571944
TAGCGCT3550.033.70250329
CTTATAC3550.033.664771
GTATGCC3700.033.647842
ATCTCCG3550.033.6406610
ATCTCGT3300.033.3168937
Produced by FastQC (version 0.11.5)