FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117240
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37010.3312627546453761No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36400.32580287136156955No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT34630.30996025921019654No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG33010.2954602413089399No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30920.27675342809065195No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30830.27594787154058215No Hit
CCCTTAATATCTTCGGAACTGGGACCAGGGTCACCGTCCTAGGTCAGCCC27670.24766388600479752No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27200.24345709068776628No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG27150.24300955927106083No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24900.22287064551931546No Hit
GTAGTAAACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGCTTCAGGGAGAACTGGTTCTTAGACGTGTCTAACGA24350.2179477999355555No Hit
CTGCTATTCCTCGCCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC23980.21463606745193514No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23540.21069779098492714No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT23110.20684902080126025No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23030.20613297053453153No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT22330.1998675307006552No Hit
GACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGATATTAAGGGTGCTG22020.19709283591708135No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGATATTAAGGGTGCTGCTGCTTGTATATGAG21360.1911854212165694No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21350.1910959149332283No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21310.19073788979986395No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20500.18348788084923562No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTACAACTATGTCTCCTGGTACCAACAACA20210.180892198632344No Hit
ACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGATATTAAGGGTGCTGC19670.17605885933192508No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19460.1741792273817622No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGATATTAAGGGTG19250.17229959543159928No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTACAACTATGTCTCCTGGTACCAACAACACCCCGATAAGGC18900.16916687551466114No Hit
ATATTAAGGGTGCTGCTGCTTGTATATGAGCTGCAGAAATAATCAGCCTC18870.16889835666463784No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18460.16522859904765314No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17020.15233969424653612No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16720.14965450574630337No Hit
GACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT16470.14741684866277613No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACCCCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15480.13855572661200816No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15260.13658658837850415No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14970.13399090616161255No Hit
GGCCTGGGCTCTGCTATTCCTCGCCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13760.12316064587734059No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13750.1230711395939995No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13190.11805878772689843No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12870.11519458665998353No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12840.11492606780996026No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12770.11429952382657263No Hit
GCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12570.1125093981597508No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12380.1108087787762701No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12200.10919766567613046No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12080.10812359027603739No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11990.10731803372596757No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11870.10624395832587448No Hit
AGTCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCG11730.10499087035909921No Hit
CTACTGGAACTGGATCCGCCAGTTCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCCATTACAGTGGGTCCAC11650.10427482009237048No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11600.10382728867566503No Hit
GTTTACTACTGTGCGGGGAGATCTTGCAGTGGTGGTGCCTGCCTGAACTGGCTCGACCCCTGGGGCCAGGGAGCC11590.10373778239232395No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11480.10275321327557195No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11400.10203716300884322No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11370.10176864415881995No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11340.10150012530879668No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11310.10123160645877341No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11210.1003365436253625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9550.042.8281441
GTTCGGT350.00109402241.13704764
GTATCAA24650.038.163091
GTGGTAT3150.037.8711851
TGGTATC3500.036.001082
ATCAACG29300.032.3073043
TCAACGC29500.031.9728494
TATCAAC29550.031.8077892
AACGCAG29900.031.663256
CAACGCA29800.031.6538075
AGAGTAC31550.030.22320611
ACGCAGA31350.030.1987637
CGCAGAG31850.029.8316088
CAGAGTA31850.029.83160810
GCAGAGT32050.029.6454499
ACGGGTC1452.7284841E-1128.1822515
GAGTTTA2700.027.77221
CTAACGA8250.027.69560869
GACGAGT500.00824050727.2440627
AATGGTA3253.6379788E-1227.03711970
Produced by FastQC (version 0.11.5)