FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243156
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28761.182779779236375No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16280.669529026633108No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC12470.512839493987399No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11410.4692460807053908No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9780.40221092631890637No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8080.332296961621346No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCG7490.3080327032851338No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7000.2878810311076017No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6950.2858247380282617No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC6740.27718830709503367No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG6650.2734869795522216No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6450.2652618072348616No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6310.2595041866127095No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC6030.24798894536840546No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT5900.24264258336212144No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5810.23894125581930942No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA5770.2372962213558374No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAG5470.22495846287979732No Hit
GAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT5470.22495846287979732No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG5410.2224909111845893No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG5260.21632203194656927No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA5170.21262070440375724No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCTAACC5050.20768560101334121No Hit
GTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCTCC5040.20727434239747322No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT4930.20275049762292519No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA4920.2023392390070572No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG4850.19946042869598118No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT4810.19781539423250916No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG4790.19699287700077317No Hit
CTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGGG4780.19658161838490515No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG4770.19617035976903716No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC4680.19246903222622513No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG4560.1875339288358091No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG4490.1846551185247331No Hit
AGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTT4470.18383260129299708No Hit
GGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGG4380.18013127375018506No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4370.17972001513431707No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC4160.17108358420108902No Hit
CCCCTACAGAATGTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGC4030.165737222194805No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC4030.165737222194805No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGC4020.16532596357893697No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT3970.16326967049959698No Hit
CTACAGAATGTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTG3930.16162463603612495No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT3920.16121337742025696No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG3850.15833456710918095No Hit
GAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATT3750.1542219809505009No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG3720.1529882051028969No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC3670.1509319120235569No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG3620.1488756189442169No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC3610.1484643603283489No Hit
GTCAATGAGGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACC3600.14805310171248087No Hit
CATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCC3580.14723058448074489No Hit
GCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTAC3560.14640806724900887No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC3520.14476303278553687No Hit
GCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCT3520.14476303278553687No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT3480.14311799832206484No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATC3440.14147296385859284No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG3400.13982792939512084No Hit
GGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG3260.1340703087729688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG3240.13324779154123279No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC3220.1324252743094968No Hit
GTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTGGAGCTG3190.1311914984618928No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA3150.12954646399842076No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA3090.12707891230321275No Hit
GGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAA3060.12584513645560874No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA3030.12461136060800473No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTCATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAG2940.12091003306519271No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.12008751583345671No Hit
GGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCG2870.11803122275411669No Hit
CGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT2860.1176199641382487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.11679744690651268No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC2820.11597492967477668No Hit
CCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTGGAG2800.11515241244304068No Hit
GTATCAACGCAGAGTACGGGTGGCAGCCCCGTCAAGGCGGGAGTGGAGAC2800.11515241244304068No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC2780.11432989521130467No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2780.11432989521130467No Hit
GTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGC2770.11391863659543668No Hit
GGTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA2770.11391863659543668No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT2750.11309611936370066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2750.11309611936370066No Hit
CTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGA2730.11227360213196466No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT2720.11186234351609665No Hit
ACCTAACCCTCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACTGT2680.11021730905262465No Hit
AGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGA2660.10939479182088864No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG2660.10939479182088864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.10692724012568063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.10528220566220862No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT2550.10487094704634062No Hit
CAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGA2510.1032259125828686No Hit
ACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCTCCCCATCCCAAGTCATC2500.10281465396700061No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG2480.1019921367352646No Hit
GGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC2470.1015808781193966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGT253.5751663E-451.602391
ACAGACC200.0095959448.377241
AGTACGT200.00960374648.36728713
AGAGTAG200.00962719448.3374511
GCTTGTA353.3771226E-546.073561
CTTGTAT407.461975E-540.2812042
GGTACGC407.461975E-540.2812048
GGGTACG407.461975E-540.2812047
GAGAGCT652.0185325E-839.76780321
CGTCACT506.0853763E-638.7017941
AACATAC350.001545264938.3341535
GGTATCA12950.037.8550341
GTATCAA32050.036.8300971
TTGTATC451.492842E-435.8055153
ACAGGTA551.1699489E-535.1834451
GGAGAGC855.6170393E-934.19790320
TCACCCT1051.0351923E-634.1974969
ATACTTG400.002757474534.0673938
CATACTT400.002871003733.78827337
TAACATA400.003032324433.41364734
Produced by FastQC (version 0.11.5)