FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863274
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29420.3407956222473977No Hit
GTATTACTGTGCGAGACGGGGCCGCGGACAATTTAATTATTACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC28550.330717709556873No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23940.2773163561047825No Hit
GTAATACAGGGCCGTATCTGCGGCGGTCACAGAGCCCAGCTTCAGGGAGA20540.23793140995790443No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20400.2363096768812683No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20220.2342245914970218No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20080.23260285842038567No Hit
CAGTAATACAGGGCCGTATCTGCGGCGGTCACAGAGCCCAGCTTCAGGGAGAACTGGTTCTTGGGCGTGTCTA19710.2283168495749901No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18600.21545882303880345No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18530.21464795650048535No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17440.20202160611810385No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16140.18696265612076815No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16000.185340923044132No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15850.18360335189059324No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15810.18313999958298294No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15800.18302416150608034No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14520.16819688766254978No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14160.16402671689405682No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT13830.1602040603562716No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13480.15614972766468121No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13300.15406464228043473No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGTTGGATTCGGCAGTC12950.15001030958884431No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12720.14734603382008493No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12650.14653516728176685No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12410.14375505343610487No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12340.1429441868977868No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12080.13993239689831966No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11850.13726812112956024No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT11370.1317078934382363No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11340.13136037920752855No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11290.13078118882301565No Hit
CTGTATTACTGTGCGAGACGGGGCCGCGGACAATTTAATTATTACTTTGA11010.12753772266974334No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11000.12742188459284076No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10920.12649517997762008No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10910.12637934190071748No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10730.12429425651647101No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10550.12220917113222453No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10540.12209333305532195No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTG10510.1217458188246142No Hit
CTCTTACACTGGGATCACCAACTACAACCCCTCCCTCAAGAGTCGAATCACCATTTCAGTAGACACGCCCAAGAA10410.12058743805558837No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10290.1191973811327574No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10280.1190815430558548No Hit
CTTTTGCCCCGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCA10190.11803900036373156No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG10080.11676478151780316No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10010.11595391497948508No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGA10000.11583807690258249No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC9940.115143048441167No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9920.11491137228736184No Hit
GTATCAGCACCGACCAGGGAAAGCCCCAAGGCTCCTCATCTTTGGTGCAT9770.11317380113382308No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9670.11201542036479728No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9640.11166790613408953No Hit
TCTTTGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCACT9520.11027784921125852No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9510.11016201113435596No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA9440.10935114459603788No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9310.10784524959630429No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9260.1072660592117914No Hit
GAGTTACAGTTCGTTGTACACTTTTGCCCCGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCAT9240.10703438305798624No Hit
GGGCAAAAGTGTACAACGAACTGTAACTCTCCTGACAATAGTAAACCGCA9230.10691854498108364No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9190.10645519267347332No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9170.10622351651966816No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9140.10587600228896041No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9060.10494929767373973No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9060.10494929767373973No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8980.10402259305851908No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT8920.1033275645971036No Hit
GGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGGTGGTGCC8900.10309588844329841No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG8840.10240085998188292No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8820.10216918382807776No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8760.10147415536666228No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8680.1005474507514416No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8670.10043161267453904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCT200.007033816552.34374248
TCGTATG2300.046.55557640
CGTATGC2350.045.621341
GTGGTAT2100.043.810581
ACGAGAC2500.043.61333522
GTATGCC2550.043.450542
ATGCCGT2550.040.8334244
GTATCAA25600.040.5970421
CACGAGA2700.040.3803821
ATCTCGT2300.040.38000537
TATACAC2800.040.145743
ATACACA2850.039.4414254
GACATCT2700.039.37858234
TAAGACA2700.039.2777931
CGTCTAC350.001435168638.9269510
CCCACGA2850.038.25287619
TACTGAA5400.037.67787670
ATCTCCG2900.037.58463310
ACAGGTA1001.0913936E-1137.482381
TTATACA3000.037.4693572
Produced by FastQC (version 0.11.5)