FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863274
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30120.34890428763057846No Hit
GTATTACTGTGCGAGACGGGGCCGCGGACAATTTAATTATTACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC28970.3355829087867815No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24090.27905392725832123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23480.2719878045672637No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22980.26619590072213456No Hit
CAGTAATACAGGGCCGTATCTGCGGCGGTCACAGAGCCCAGCTTCAGGGAGAACTGGTTCTTGGGCGTGTCTA18560.21499547073119313No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18330.21233119496243372No Hit
GTAATACAGGGCCGTATCTGCGGCGGTCACAGAGCCCAGCTTCAGGGAGA17420.2017899299642987No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16220.1878893607359888No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16200.18765768458218365No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15980.18510924689032685No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15090.17479965804599698No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14720.17051364920060144No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14600.16912359227777043No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13870.16066741266388193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13150.15232707112689597No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12690.1469985195893772No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGTTGGATTCGGCAGTC12690.1469985195893772No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12440.14410256766681262No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12320.14271251074398164No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12150.14074326343663773No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12120.14039574920592998No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11860.13738395920646285No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11730.13587806420672927No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11570.13402465497628796No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11550.13379297882248278No Hit
CTCTTACACTGGGATCACCAACTACAACCCCTCCCTCAAGAGTCGAATCACCATTTCAGTAGACACGCCCAAGAA11210.12985448420779497No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10960.1269585322852304No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10950.12684269420832783No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10910.12637934190071748No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10560.12232500920912712No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10210.11827067651753673No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT10180.11792316228682898No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10090.11688061959470573No Hit
CTTTTGCCCCGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCA10020.11606975305638766No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9840.11398466767214117No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9840.11398466767214117No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9740.11282628690311536No Hit
CTGTATTACTGTGCGAGACGGGGCCGCGGACAATTTAATTATTACTTTGA9700.112362934595505No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGA9660.11189958228789469No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9650.11178374421099212No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9560.11074120151886888No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9310.10784524959630429No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9250.1071502211348888No Hit
GGGCAAAAGTGTACAACGAACTGTAACTCTCCTGACAATAGTAAACCGCA9240.10703438305798624No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGCACGTATTTACAATGGTATCAGCACCGA9230.10691854498108364No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9210.10668686882727849No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC9200.10657103075037588No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9170.10622351651966816No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9150.10599184036586297No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTG9140.10587600228896041No Hit
TCTTTGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCACT9090.10529681190444748No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9030.104601783443032No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8990.10413843113542165No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8860.10263253613568808No Hit
GAGTTACAGTTCGTTGTACACTTTTGCCCCGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCAT8800.10193750767427259No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8790.10182166959737No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8750.10135831728975968No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8700.10077912690524676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)