FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797680
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC29760.37308193761909536No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT29480.3695717580984856No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29330.36769130478387324No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28140.3527730418212817No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25770.32306187945040615No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24530.3075167987162772No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT24240.3038812556413599No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24160.3028783472069No Hit
ATAATAATCCGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG22900.287082539364156No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22850.2864557215926186No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21010.2633888276000401No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19280.24170093270484402No Hit
GTATACATAGTTCCTCCCGATGTTGGAGCTGTTTCCAAAACAAGAGATGGTGACGCTCTGCCCGGGGGTCCCAGA18980.2379400260756193No Hit
GATTATTATTGTGCAGCGTGGGATGACAGTGGTCGGGGAATCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGT18970.2378146625213118No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGATTCCCCGACCACTGTCATCCCACGCTGCA18960.23768929896700433No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGCGTCAC18790.23555811854377695No Hit
GGATGACAGTGGTCGGGGAATCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGTCAGCCCAAGGCCAACCCCA17720.22214421823287533No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17460.21888476582088054No Hit
CTATGTATACTGGTACCAGCAGTTCCCAGGAACTGCCCCCAAACTCGTCA16170.20271286731521412No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16100.20183532243506166No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15570.19519105405676462No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15450.19368669140507472No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15340.19230769230769232No Hit
CTATAGATGACGAGTTTGGGGGCAGTTCCTGGGAACTGCTGGTACCAGTATACATAGTTCCTCCCGATGTTGGAG15010.18817069501554506No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14790.18541269682078027No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGATTCCCCGACCA14590.18290542573463042No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14160.17751479289940827No Hit
GTATCAACGCAGAGTACGGGAGCTTCAACTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG13800.17300170494433859No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGATTCCCCGACCACTGTCATCCCACGCTGCACAATAATAATC13300.16673352722896398No Hit
GCACAATAATAATCCGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG12610.15808344198174706No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12580.15770735131882457No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12530.15708053354728713No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT11820.14817972119145523No Hit
GAACTATGTATACTGGTACCAGCAGTTCCCAGGAACTGCCCCCAAACTCGTCATCTATAGTGATCATCAGCGGCC11810.14805435763714772No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11580.14517099588807542No Hit
GTAATAAGTGGCCGTGTCTTCAGACATCAGACCGTTCAGCTCCATGTAGATTGTATTCGTGGACATGTCGGTGGA11500.1441680874536155No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11340.1421622705846956No Hit
GAGTACGGGAGCTTCAACTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT11200.14040718082439074No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11100.13915354528131582No Hit
ATGTATACTGGTACCAGCAGTTCCCAGGAACTGCCCCCAAACTCGTCATC11060.13865209106408585No Hit
GACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGATTCCCCGACCACTGTCATCCCACGCTGCACAATAATAAT10910.13677163774947346No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10830.13576872931501355No Hit
ACATAGTTCCTCCCGATGTTGGAGCTGTTTCCAAAACAAGAGATGGTGACGCTCTGCCCGGGGGTCCCAGACG10620.1331360946745562No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10260.1286230067194865No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10220.12812155250225654No Hit
GTACGGGAGCTTCAACTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC10090.12649182629625916No Hit
CATCTATAGTGATCATCAGCGGCCCTCAGACATCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC10030.1257396449704142No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC9970.12498746364456925No Hit
CTGTCATCCCACGCTGCACAATAATAATCCGCCTCATCCTCGGACCGGAG9930.12448600942733928No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGCGTCACCATCTCTTGTTTTGGAAACAGCTCCAACATCGGGAGGAA9920.1243606458730318No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9500.11909537659211714No Hit
TTATTGTGCAGCGTGGGATGACAGTGGTCGGGGAATCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGTCAGCC9330.11696419616888977No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9310.1167134690602748No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9290.11646274195165982No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9290.11646274195165982No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9260.11608665128873734No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCC9200.1153344699628924No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9110.11420619797412497No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCGGATTATTATTGTGCAGCGTGGGATGACAGTGGTCGGGGAAT9080.1138301073112025No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT8990.11270183532243506No Hit
GTCCGAGGATGAGGCGGATTATTATTGTGCAGCGTGGGATGACAGTGGTC8860.11107210911643767No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8860.11107210911643767No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG8730.10944238291044027No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8410.10543074917260054No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGCCAGGGATGTCTGAGGGCCGCTG8390.10518002206398556No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG8350.1046785678467556No Hit
TCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCC8220.1030488416407582No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8220.1030488416407582No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8170.10242202386922074No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGC8040.10079229766322335No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8030.10066693410891586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2850.059.9389670
TATACAC2150.052.2401163
TCGTATG2050.052.1378940
CGTATGC2150.051.4013841
CACGAGA2150.050.66026321
CGAGACA2250.049.92772323
ATCTCGT1900.048.81875637
TTATACA2400.046.7984432
TCTCGTA2000.046.43657738
ACGAGAC2350.046.3487522
GCAATCT2000.046.2607434
CTTATAC2450.045.8721281
TCTCCGA2450.045.8433711
ATCTCCG2500.044.926510
ATGCCGT2500.044.40855444
GTGGTAT2400.043.9897461
TGGTATC2400.043.9621732
GGTATCA8600.043.9567451
AGGCAAT2500.043.7846632
GGCAATC2150.043.0088233
Produced by FastQC (version 0.11.5)